Preparative Chromatography and Downstream Processing
نویسندگان
چکیده
منابع مشابه
Preparative Supercritical Fluid Chromatography
Supercritical Suid chromatography (SFC) on a preparative scale is of interest because of its advantages over high performance liquid chromatography (HPLC). The high diffusivity and low viscosity of supercritical Suids allow rapid separation. The solvent strength can easily be controlled by changing pressure and/or temperature, so that mobile phases with a Rxed composition can be used to separat...
متن کاملLarge-Scale, Preparative Paper Chromatography
Most of the techniques that have been described for large-scale, preparative paper c hromatograp hy depe nd on an i.ncrease in bulk through the use of many closely pressed layers of a thin paper [IJ.' So me of these methods require the tedious loading of each s hee t , and others tend to suffer from a lack of uniformity in the movement of compounds thro ughout the bulk. Th e latter disadvantage...
متن کاملPreparative Thin-Layer (Planar) Chromatography
The various OPLC andRPC techniques are compared in Table 1. Study of the data shows that OPLC is an excellent technique for analytical separations and that RPC is more ideally suited as a preparative method for isolation of compounds from biological matrices. The advantage of combining online and ofSine separations and two-dimensional development can also be exploited in OPLC. The advantage of ...
متن کاملMultimodal chromatography: an efficient tool in downstream processing of proteins.
Chromatography has become an indispensable tool for the purification of proteins. Since the regulatory demands on protein purity are expected to become stricter, the need for generating improved resins for chromatographic separations has increased. More advanced scientific investigations of protein structure/function relationships, in particular, have also been a driving force for generating mo...
متن کاملMultimodal chromatography: debottlenecking the downstream processing of monoclonal antibodies
2015 The commercial potential of monoclonal antibodies (mAbs) has been continuously increasing during the last years alongside with the number of approved mAb-based drugs and clinical trials. Despite their effectiveness and safety, the general access to this class of biopharmaceuticals is barred by high selling prices. Downstream processing is now considered the bottleneck in the manufacturing ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical Engineering & Technology
سال: 2011
ISSN: 0930-7516
DOI: 10.1002/ceat.201190081